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TEV Protease - an overview | ScienceDirect Topics

TEV Protease - an overview | ScienceDirect Topics

https://www.sciencedirect.com/topics... 

Tobacco etch virus (TEV) protease with multiple mutations ...

Tobacco etch virus (TEV) protease with multiple mutations ...

https://febs.onlinelibrary.wiley.com... 

TEV Protease, His - GenScript

TEV Protease, His - GenScript

https://www.genscript.com/protein/Z0... 

TEV Protease | NEB

The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ S; however, the amino acid in the P1’ position can also be G, A, M, C, or H (1). It is often used for the removal of affinity purification tags such as maltose...

https://international.neb.com/produc... 

TEV protease - Wikipedia

The preferred, native cleavage sequence was first identified by examining the cut sites in the native polyprotein substrate for recurring sequence. The consensus for these native cut sites is ENLYFQ\S where ‘\’ denotes the cleaved peptide bond...

https://en.wikipedia.org/wiki/TEV_pr... 

TEV Protease, His - GenScript

Recombinant Tobacco Etch Virus (TEV). The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln- (Gly/Ser) [ENLYFQ (G/S)] and cleavage occurs between the Gln and Gly/Ser residues, The most commonly used sequence is ENLYF...

https://www.genscript.com/protein/Z0... 

TEV Protease FAQ - Johns Hopkins University

TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokin...

https://pages.jh.edu/fleming/Local/P... 

TEV protease cutting site - ResearchGate

Jan 01, 1993  · TEV protease recognizes the following peptide sequence E-X-X-Y -X-Q- (G/S) , where X can be any amino acid. The cleavage occurs betwen QG or QS . The most common TEV protease cleavage sequence used...

https://www.researchgate.net/post/TE... 

Linker sequences to add between TEV site and protein?

We have a mammalian cell expression protein, want to add the TEV cleavage site at the C-terminal, have given the ORF sequence in the oligoperfect designer, and have given TEV site, after checking ...

https://www.researchgate.net/post/Li... 

Addgene: pcDNA3.1 TEV (full-length)

Plasmid pcDNA3.1 TEV (full-length) from Dr. Xiaokun Shu's lab contains the insert Tobacco etch virus (TEV) protease and is published in Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 10.1073/pnas.1502857112. Epub 2015 Mar 2. This plas...

https://www.addgene.org/64276/ 

RCSB PDB - 1Q31: Crystal Structure of the Tobacco Etch ...

Jul 28, 2003  · Tobacco etch virus (TEV) protease is a cysteine protease exhibiting stringent sequence specificity. The enzyme is widely used in biotechnology for the removal of the affinity tags from recombinant fusion proteins.

https://www.rcsb.org/structure/1q31 

p1 protease - Tobacco etch virus (TEV)

Oct 03, 2006  · PS51871 , PV_P1_PRO, 1 hit. This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence (s), incl...

https://www.uniprot.org/uniprot/Q0GD... 

pENTR™ Directional TOPO® Cloning Kits

pENTR™/TEV/D-TOPO® Contains a Tobacco Etch Virus (TEV) recognition site for efficient TEV protease-dependent cleavage of an N-terminal tag from your recombinant protein after recombination and expression from a Gateway® destination vec...

https://content.fccc.edu/yen/docs/pe... 

pENTR TEV D-TOPO Sequence and Map

pENTR™/TEV/D-TOPO® Directional TOPO® cloning vector with a TEV protease site, for creating a Gateway® entry vector that is suitable for recombination with a destination vector.

https://www.snapgene.com/resources/p... 

What is TEV sequence? – Moorejustinmusic.com

Jun 03, 2019  · What is TEV sequence? TEV protease (EC 3.4. 22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV). Due to its high sequence specifi...

https://moorejustinmusic.com/student... 

TEV Protease, Recombinant*

4°C (table 1). Following digestion, TEV Protease can be removed from the reaction via the polyhistidine tag sequence by affinity chromatography. Components: 10127-017 TEV Protease, Recombinant Y02233 20X rTEV Buffer Y00147 0.1 M DTT Store rTE...

https://tools.thermofisher.com/conte... 

TEV Protease His6

Mar 07, 2018  · Properties of the Protean TEV Protease His6 It is highly specific and active for its seven-amino acid sequence with minimal off-target effects. It has activity more than 10,000 units per 1 mg of protein. The activity...

https://www.protean.bio/en/article/t... 

Addgene: 6xHis-TEV-UBC-9

Plasmid 6xHis-TEV-UBC-9 from Dr. Ronald Hay's lab contains the insert UBC-9 and is published in Nat Commun. 2014 Dec 5;5:5485. doi: 10.1038/ncomms6485. This plasmid is available through Addgene.

https://www.addgene.org/87055/ 

Expression and purification of soluble His(6)-tagged TEV ...

This chapter describes a simple method for overproducing a soluble form of the tobacco etch virus (TEV) protease in Escherichia coli and purifying it to homogeneity so that it may be used as a reagent for removing affinity tags from recombinant pr...

https://pubmed.ncbi.nlm.nih.gov/1898... 

TEV Protease | Gene And Cell Technologies

The protease from Tobacco Etch Virus (TEV) is a highly sequence-specific endoprotease that can be used for cleaving target proteins in defined places in vitro or in vivo. Its most efficient recognition sequence is ENLYFQ*G/S(whereas the * symboliz...

https://www.geneandcell.com/products... 

Vector information sheet

Sequence accession/link (SGC) Description pGEX expression vector with N -terminal GST tag and TEV protease cleavage site. Includes sites for LIC cloning, and a “stuffer” fragment that includes the SacB gene, allowing negative selection on 5% s...

https://www.thesgc.org/sites/default... 

FOR RESEARCH USE ONLY! EZCut™ TEV Protease, Recombinant

protein that enables access of the TEV recognition sequence by the enzyme. The target fusion protein (1 mg/ml) containing TEV protease cleavage site should be purified to homogeneity. Recommended cleavage buffer is 50 mM Tris, buffer, 0.1 M NaCl, ...

https://www.biovision.com/documentat... 

Can TEV Protease recognize and cleave a sequence other ...

Jul 06, 2018  · FAQ: Can TEV Protease recognize and cleave a sequence other than Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)? The TEV Protease recognition sequence with the highest catalytic efficiency is ENLYFQ S. However, the amino acid in ...

https://international.neb.com/faqs/2... 

Cleavable C-terminal His-tag vectors for structure ...

When expressed in pMCSG26, the protein encoded by this clone includes the TEV protease recognition sequence in addition to the His-tag (in total, -ENLYFQSAGHHHHHH) at the C-terminus. Subsequent cleavage with TEV protease will leave the first six r...

https://www.ncbi.nlm.nih.gov/pmc/art... 

Primers/Plasmids/PCR sequences - 2008.igem.org

TEV Protease cut site. Amino Acid Sequence : Glu-Asn-Leu-Tyr-Phe-Gln-Gly (Cuts between Gln-Gly) Base Pair Sequence: GAAAACCTGTACTTCCAGGGT P3: Forward Primer for GFP. forward sequence: GGC GGC AGC GGC GGG GGC AGC ATG CGT AAA GGA GAA GAA C length = ...

http://2008.igem.org/Primers/Plasmid... 

TEV Protease Histidine-tagged recombinant, expressed in E ...

TEV polyprotein: P1, HC-Pro, and Nuclear Inclusion a (NIa). In its native form, TEV protease is a 27 kDa catalytic domain that was originally noted as the C-terminal portion of the NIa part of the TEV polyprotein.1,2 TEV protease specifically clea...

https://www.urmc.rochester.edu/Media... 

Directed evolution improves the catalytic efficiency of ...

Dec 09, 2019  · Tobacco etch virus protease (TEV) is one of the most widely used proteases in biotechnology because of its exquisite sequence specificity. A limitation, however, is its …

https://www.nature.com/articles/s415... 

pMAL-c6T Vector | NEB

The vector expresses an N-terminal hexahistidine tagged malE gene (lacking its secretory signal sequence) followed by a multiple cloning site containing a TEV protease recognition sequence and stop codons in all three frames. This allows MBP to be...

https://www.neb.com/products/n0378-p... 

TEV Protease | Sigma-Aldrich

TEV protease has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln↓Gly/Ser. Features and Benefits Optimally engineered and purified for enhanced stability and high specific activity over a broad temperature range. Ph...

https://www.sigmaaldrich.com/US/en/p... 

TEV Protease - an overview | ScienceDirect Topics

Apr 02, 2010  · In the original Tango design, the TEV protease and actuator module (tetracycline transactivator protein—tTa) with its TEV protease cleavage site were fused to an adaptor beta-arrestin and the C-terminus of a GPCR, ...

https://www.sciencedirect.com/topics... 

Efficient site-specific processing of fusion proteins by ...

The tobacco etch virus (TEV) protease is the best-characterized enzyme of this type. TEV protease cleaves the sequence ENLYFQG/S between QG or QS with high specificity. The tobacco vein mottling virus (TVMV) protease is a close relative of TEV pro...

https://pubmed.ncbi.nlm.nih.gov/1547... 

Gene design, fusion technology and TEV cleavage conditions ...

Jan 17, 2017  · Tobacco etch virus (TEV) protease is one of the most popular enzymes used to remove fusion tags from recombinant proteins due to the stringent sequence specificity it displays. However, TEV protease may require a Gly...

https://microbialcellfactories.biome... 

TEV Protease (T4455) - Product Information Sheet

N-terminus of the TEV polyprotein: P1 HC-Pro Nuclear Inclusion (NI ) (e.g. In its native form, TEV protease is a 27 kDa catalytic domain that was originally noted as the C-terminal portion of the NI part of the TEV polyprotein.1,2 TEV protease spe...

https://www.sigmaaldrich.com/deepweb... 

Harms Lab | Cloning of S100 with N-terminal TEV cleavage ...

Sep 03, 2014  · In order to make the histidine-tag removable with a TEV digestion, we need to insert the TEV cleavage site (E-N-L-Y-F-Q-S) between the 6xHis-tag and the start codon of the S100 gene. This is also done with PCR. To cr...

https://harmslab.uoregon.edu/wiki/ha... 

TEV Protease (His Tag)-Gene Universal

TEV Protease (Tobacco etch virus protease )is a recombinant protease derived from the Tobacco etch virus (TEV) Nla, which is used to remove the affinity labeling of the purified fusion protein.TEV Protease has a strong site specificity, which can ...

https://www.geneuniversal.com/index.... 

Tobacco etch virus (TEV) protease with multiple mutations ...

Mar 03, 2020  · Tobacco etch virus (TEV) protease is a 27-kDa catalytic domain of the polyprotein nuclear inclusion a (NIa) in TEV, which recognizes the specific amino acid sequence ENLYFQG/S and cleaves between Q and G/S. Despite i...

https://febs.onlinelibrary.wiley.com... 

Construction and use of new cloning vectors for the rapid ...

site as a 50 cloning site results in four additional residues (Gly-Ala-Ser-His) at the N-terminus of the protein after TEV protease cleavage (Fig. 1). While the use of this site adds several additional residues, the NdeI site is a common 50 clonin...

https://biochem.wisc.edu/sites/defau... 

Directed evolution improves the catalytic efficiency of ...

Despite the exquisite sequence-specificity of TEV, a major limitation is its slow catalysis. With a k cat of 0.18 s −1 (for its best high-affinity substrate sequence, ENLYFQS ), TEV is slower than many other proteases used for biotechnology, suc...

https://www.ncbi.nlm.nih.gov/pmc/art... 

Biochemical and mutational analysis of a plant virus ...

sequence divergence was observed between positions +5 and +10. Therefore, the primary sequence analysis ofa cleavage site in 10 different TEV isolates supported the hypothesis that the sequence . . . Glu-Xaa-Xaa-Tyr-Xaa-Gln-Ser or Gly . . . is req...

https://www.embopress.org/doi/epdf/1... 

Purification of His-Tagged Protein - CSGID

(TEV protease to cut the tag) Day 1 •Thaw cells in ice water, add Protein Inhibitor Cocktail (PIC) (0.5-0.25 mM final concentration) Sonicate, add more PIC (1 mM) •Pour cell lysate into centrifuge tubes, balance, spin 17,000 rpm, at 4 ºC,...

https://csgid.org/csgid/data/protoco... 

AcTEV™ Protease - US

AcTEV™ Protease specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), making it useful for removing affinity tags from fusion proteins. AcTEV™ Protease is an improved version of Tobacc...

https://www.thermofisher.com/order/c... 

pET28-SBP-TEV Vector

SBP -tag followed by TEV cleavage site . Two stop codons are included in the vector at the C -terminal cloning site . Antibiotic resistance Kanamycin Promoter T7 - lacO Cl oning Methods Insertion of a DNA sequence into the cloning/expression regio...

https://www.thesgc.org/sites/default... 

DNASU Plasmid | Detailed Vector Information: pet16B-TEV

TEV protease cleavage site 5359 5382 repressor binding site lac operator ... trxn termination sequence T7 term T7 terminator 5456 5502 DNASU Plasmid Repository • PSI:Biology-Materials Repository. The Biodesign Institute/Arizona State University ...

http://dnasu.org/DNASU/GetVectorDeta... 

TEV Cleavage Site Antibody (PA1-119)

The membrane was probed with a TEV cleavage site polyclonal antibody (Product # PA1-119) at a dilution of 1:500 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG-HRP secondary antibody ...

https://www.thermofisher.com/antibod... 

Tev Protease Cleavage Site Enlyfq | Thermo Fisher | Bioz

Thermo Fisher tev protease cleavage site enlyfq Thermo Scientific WELQut Protease is highly specific recombinant serine protease of Staphylococcus aureus It recognizes and precisely cleaves recombinant proteins containing an engineered recognition...

https://bioz.com/result/tev%20protea... 

A Beginner’s Guide to Tag-Removing Proteases

Sep 28, 2016  · TEV Protease: TEV protease has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln-Gly, and cleaves before the Gly. TEV protease has been fused to a number of other affinity tags, including...

https://bitesizebio.com/30294/beginn... 

Recombinant TEV Protease His (N-Term) Protein (NBP2-61441 ...

TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. In this form X and X' stand for any of the amino acid residues, except that X' cannot be P. The optimal cleavage site is ENLYFQ/G. A...

https://www.novusbio.com/products/te... 

EZCut™ TEV Protease, Recombinant | 7847 | BioVision, Inc.

BioVision’s EZCut™ TEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly/Ser. The optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser/Glycine (ENLYFQS/G). It cont...

https://www.biovision.com/ezcuttm-te... 

EXPRESSION AND PURIFICATION OF THE TRANSMEMBRANE ...

TEV protease recognition site’s protein sequence.....87 U92C Sel K’s protein sequence.....87 . vi i LIST OF TABLES Table 3.1 Detergents, with family classification, used in the TEV protease ... Figure 2.10 The PCR cycle parameters for the TEV ...

https://udspace.udel.edu/bitstream/h... 

Expression and Purification of Soluble His6-Tagged TEV ...

 · Summary. This chapter describes a simple method for overproducing a soluble form of the tobacco etch virus (TEV) protease in Escherichia coli and purifying it to homogeneity so that it may be used as a reagent for removing affini...

https://link.springer.com/protocol/1... 

Vector | CellFree Sciences

The world's largest manufacturer of wheat germ cell-free protein expression system products. We are the only company in the world that provides both products and custom protein expression/production services specialized in wheat germ cell-free pro...

https://www.cfsciences.com/eg/produc... 

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